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This Article Full Text Full Text (PDF) Supplementary data All Versions of this Article: JOE-08-0175v1 198/3/607    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Google Scholar Articles by van Herp, F. Articles by Martens, G. J M PubMed PubMed Citation Articles by van Herp, F. Articles by Martens, G. J M

Department of Molecular Animal Physiology, Faculty of Science, Donders Center for Neuroscience, Nijmegen Center for Molecular Life Sciences (NCMLS), Radboud University, Geert Grooteplein Zuid 28, 6525 GA Nijmegen, The Netherlands¹ Department of Environment and Agrobiotechnologies, Centre de Recherche Public-Gabriel Lippmann, L-4422 Belvaux, Luxembourg² Laboratory of Protein Biochemistry and Biomolecular Engineering, Ghent University, B-9000 Ghent, Belgium

(Correspondence should be addressed to F van Herp; Email: f.vanherp{at}ncmls.ru.nl) www.ru.nl/molanphys/

To study in vivo the dynamics of the biosynthetic and secretory processes in a neuroendocrine cell, we use the proopiomelanocortin-producing intermediate pituitary melanotrope cells of Xenopus laevis. The activity of these cells can be simply manipulated by adapting the animal to a white or a black background, resulting in inactive and hyperactive cells respectively. Here, we applied differential display proteomics and field emission scanning electron microscopy (FESEM) to examine the changes in architecture accompanying the gradual transition of the inactive to the hyperactive melanotrope cells. The proteomic analysis showed differential expression of neuroendocrine secretory proteins, endoplasmic reticulum (ER)-resident chaperones, and housekeeping and metabolic proteins. The FESEM study revealed changes in the ultrastructure of the ER and Golgi and the number of secretory granules. We conclude that activation of neuroendocrine cells tunes their molecular machineries and organelles to become professional secretors.