HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: JOE-08-0027v1 198/3/489    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Sakai, N. Articles by Sakurada, K. PubMed PubMed Citation Articles by Sakai, N. Articles by Sakurada, K.

Department of Stem Cell Based Drug Discovery, Research Center Kobe, Bayer Yakuhin Ltd, Kobe, Hyogo 650-0047, Japan¹ Division of Sex Differentiation, National Institute for Basic Biology, Myodaiji-cho, Okazaki, Nagoya 444-8787, Japan² Laboratory of Molecular Pharmacology, Biosignal Research Center, Kobe University, Kobe, Hyogo 657-8501, Japan³ Department of Systems Biomedicine, National Research Institute for Child Health and Development, 157-8535 Tokyo, Japan

(Correspondence should be addressed to K Sakurada; Email: kazuhiro.sakurada{at}gmail.com)

^* (N Sakai and H Terami contributed equally to this work)

Nuclear receptor subfamily 5, group A, member 1 (NR5A1 previously known as SF-1/AD4BP) is a transcription factor involved in the development of adrenal/gonadal tissues and steroidogenic linage cell differentiation in adult somatic stem cells. To understand the cellular signaling network that regulates NR5A1 gene expression, loss of function screening with an siRNA kinome library, and gain of function screening with an addressable full-length cDNA library representing one quarter of the human genome was carried out. The NR5A1 gene expression was activated in mesenchymal stem cells by siRNA directed against protein kinase C (PKC)-, erb-B3, RhoGAP (ARHGAP26), and hexokinase 2, none of which were previously known to be involved in the NR5A1 gene expression. Among these, we identified crosstalk between erb-B3 and PKC- signaling cascades. In addition, the gain of function studies indicated that sex-determining region Y (SRY)-box 15 (SOX15), TEA domain family member 4, KIAA1257 (a gene of unknown function), ADAM metallopeptidase with thrombospondin type 1 motif 6, Josephin domain containing 1, centromere protein, TATA box-binding protein-associated factor 5-like RNA polymerase, and inducible T-cell co-stimulator activate NR5A1 gene expression. These results provide new insights into the molecular mechanisms of NR5A1 gene expression.