JOE
HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Journal of Endocrinology (2007) 195, 407-414       DOI: 10.1677/JOE-07-0436
© 2007 Society for Endocrinology
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (13)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhao, M.
Right arrow Articles by Huang, G. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhao, M.
Right arrow Articles by Huang, G. C.

Evidence for the presence of stem cell-like progenitor cells in human adult pancreas

 Min Zhao, Stephanie A Amiel, Michael R Christie, Paolo Muiesan1, Parthi Srinivasan1, Wendy Littlejohn1, Mohamed Rela1, Matthew Arno2, Nigel Heaton1 and Guo Cai Huang

Department of Diabetes, Endocrinology and Internal Medicine, King's College London School of Medicine, Bessemer Road, London SE5 9PJ, UK 1 Institutes of Liver Studies, King's College London School of Medicine, London SE5 9PJ, UK 2 School of Biomedical and Health Sciences, King's College London, Genomics Centre, London SE1 9NH, UK

(Correspondence should be addressed to G C Huang; Email: guo.huang{at}kcl.ac.uk)

The origin of cells replacing ageing ß-cells in adult life is unknown. This study assessed the expression of classic stem cell markers: Oct4, Sox2 and CD34 in islet-enriched fractions versus exocrine cell-enriched fractions from 25 adult human pancreases following human islet isolation. Expression of Oct4, Sox2 and CD34 mRNAs was found in all cell samples, with no significant differences between endocrine and exocrine cell fractions. Immunohistochemical staining for Oct4, Sox2, CD133, CD34, CK19, insulin and nestin on human pancreas sections showed that the majority of Oct4+ve cells were found in the walls of small ducts. Similar localisations were observed for Sox2+ve cells. The majority of Sox2+ve cells were found to co-express Oct4 proteins, but not vice versa. Cells positive for Oct4 and Sox2 appeared to be a unique cell population in the adult human pancreases without co-expression for CK19, CD34, CD133, insulin and nestin proteins. The numbers of Oct4+ve and Sox2+ve cells varied among donors and were ~1–200 and 1–30 per 100 000 pancreatic cells respectively.




This article has been cited by other articles:


Home page
 PhysiologyHome page
S. Z. Young and A. Bordey
GABA's Control of Stem and Cancer Cell Proliferation in Adult Neural and Peripheral Niches
Physiology, June 1, 2009; 24(3): 171 - 185.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
A. Di Bella, M. Regoli, C. Nicoletti, L. Ermini, L. Fonzi, and E. Bertelli
An Appraisal of Intermediate Filament Expression in Adult and Developing Pancreas: Vimentin Is Expressed in {alpha} Cells of Rat and Mouse Embryos
J. Histochem. Cytochem., June 1, 2009; 57(6): 577 - 586.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
T. Guo and M. Hebrok
Stem Cells to Pancreatic {beta}-Cells: New Sources for Diabetes Cell Therapy
Endocr. Rev., May 1, 2009; 30(3): 214 - 227.
[Abstract] [Full Text] [PDF]


HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2007 by the Society for Endocrinology.