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Journal of Endocrinology (2007) 194, 393-406    DOI: 10.1677/JOE-07-0085
© 2007 Society for Endocrinology
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Molecular cloning and expression analysis of rainbow trout (Oncorhynchus mykiss) CCAAT/enhancer binding protein genes and their responses to induction by GH in vitro and in vivo

 Jay H Lo, Pinwen Peter Chiou, C M Lin and Thomas T Chen

Department of Molecular and Cell Biology, University of Connecticut, 91 N. Eagleville Road, U-3125, Storrs, Connecticut 06269, USA

(Correspondence should be addressed to T T Chen; Email: thomas.chen{at}uconn.edu)

CCAAT/enhancer-binding proteins (C/EBPs) are transcription factors consisting of six isoforms and play diverse physiological roles in vertebrates. In rainbow trout (Oncorhynchus mykiss), in addition to the reported C/EBPß1, we have isolated cDNA of four other isoforms, C/EBP{alpha}, C/EBPß2, C/EBP{delta}1, C/EBP{delta}2, from the liver. Comparison of the deduced amino acid sequence of rainbow trout C/EBPs with those of other vertebrates revealed that C/EBP isoforms are highly conserved. The profiles of tissue-specific expression of individual C/EBP isoform mRNA, determined by quantitative real-time (RT)-PCR showed distinct patterns. Furthermore, injection of bovine GH into yearling rainbow trout resulted in a significant increase of mRNA levels of C/EBPß1, C/EBPß2, and C/EBP{delta}2 but not C/EBP{alpha} and C/EBP{delta}1 in the liver. GH-dependent increase of mRNA levels of C/EBPß1, C/EBPß2, C/EBP{delta}2, and IGF-II were also confirmed by treating rainbow trout hepatoma cells expressing a goldfish GH receptor with bGH. Together with our previous findings, the results presented in this paper strengthen our previous hypothesis that GH may regulate the expression of the IGF-II gene via mediating the expression of C/EBPß1, C/EBPß2, and C/EBP{delta}2 mRNA.






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