HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Data Table 1 Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (3) Citing Articles via Google Scholar Google Scholar Articles by Corless, M. Articles by Newsholme, P. Search for Related Content PubMed PubMed Citation Articles by Corless, M. Articles by Newsholme, P.

School of Biomolecular and Biomedical Sciences, Conway Institute, University College Dublin, Belfield, Dublin 4, Ireland
¹ School of Biomedical Sciences, University of Ulster, Coleraine, Northern Ireland, UK

(Requests for offprints should be addressed to P Newsholme; Email: philip.newsholme{at}ucd.ie)

Supplementary material
Supplementary data table 1 associated with this article can be found in the online version of Journal of Endocrinology at http://joe.endocrinology-journals.org/content/vol190/issue3.

We have investigated the effects of prolonged exposure (24 h) to the amino acid L-glutamine, on gene and protein expression using clonal BRIN-BD11 ß-cells. Expression profiling of BRIN-BD11 cells was performed using oligonucleotide microarray analysis. Culture for 24 h with 10 mM L-glutamine compared with 1 mM resulted in substantial changes in gene expression with 148 genes upregulated more than 1.8-fold, and 18 downregulated more than 1.8-fold, including many genes involved in cellular signaling, metabolism, gene regulation, and the insulin-secretory response. Subsequent functional experiments confirmed that L-glutamine increased the activity of the Ca²⁺ regulated phosphatase calcineurin and the transcription factor Pdx1. Additionally, we demonstrated that ß-cell-derived L-glutamate was released into the extracellular medium at high rates. As calcineurin is a regulator of the glutamate N-methyl-D-aspartate (NMDA) receptor activity, we investigated the action of NMDA on nutrient-induced insulin secretion, and demonstrated suppressed insulin release. These observations indicate important long-term effects of L-glutamine in regulating ß-cell gene expression, signaling, and secretory function.

This article has been cited by other articles:

				A. Kiely, N. H McClenaghan, P. R Flatt, and P. Newsholme Pro-inflammatory cytokines increase glucose, alanine and triacylglycerol utilization but inhibit insulin secretion in a clonal pancreatic {beta}-cell line J. Endocrinol., October 1, 2007; 195(1): 113 - 123. [Abstract] [Full Text] [PDF]

				N. H. McClenaghan Physiological regulation of the pancreatic {beta}-cell: functional insights for understanding and therapy of diabetes Exp Physiol, May 1, 2007; 92(3): 481 - 496. [Abstract] [Full Text] [PDF]