| HOME | HELP | CONTACT US | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
reversibly disrupts the blood–testis barrier and impairs Sertoli–germ cell adhesion in the seminiferous epithelium of adult rat testes
Population Council, 1230 York Avenue, New York 10021, USA
1 Department of Zoology, The University of Hong Kong, Hong Kong, China
(Requests for offprints should be addressed to C Y Cheng; Email: y-cheng{at}popcbr.rockefeller.edu)
* (M W M Li, W Xia, D D Mruk, C Q F Wang, H H N Yan and M K Y Siu contributed equally to this work)
The timely restructuring of the blood–testis barrier (BTB) that facilitates the migration of preleptotene and leptotene spermatocytes from the basal to the adluminal compartment in the seminiferous epithelium of adult rat testes, which occurs at late stage VII through early stage VIII of the epithelial cycle, is a crucial cellular event of spermatogenesis. However, the regulation of BTB dynamics at the biochemical level remains elusive. In this study, tumor necrosis factor 
(TNF), a secretory product of Sertoli and germ cells in rat testes, was shown to affect junction dynamics in vivo. Following an acute administration of recombinant TNF directly to adult rat testes in vivo at 0.5 and 2 µg/testis (with a body weight ~300 g), this treatment significantly and transiently disrupted the BTB. It also transiently inhibited the steady-state protein levels of occludin, zonula occludens-1, and N-cadherin, but not junction adhesion molecule-A, -, and ß-catenin in testes at the BTB site as illustrated by immunoblottings, immunohistochemistry, electron microscopy, and fluorescent microscopy. This transient disruption of the BTB integrity induced by TNF treatment was further demonstrated by a functional test to assess the passage of a fluorescent dye (e.g. fluorescein-5-isothiocyanate) from the systemic circulation to the adluminal compartment. Additionally, both the phosphorylated-Ser/Thr protein kinase activated by MAP kinase kinase (p-p38) and phosphorylated-externally regulated kinase (p-ERK) mitogen -activated protein kinase-signaling pathways were transiently activated. Collectively, these data coupled with the recently published in vitro studies have illustrated that the BTB is likely utilizing a novel mechanism in which localized production of TNF by Sertoli and germ cells into the microenvironment at the basal compartment facilitates the timely restructuring (‘opening’?) of the BTB during spermatogenesis to facilitate germ cell migration.
This article has been cited by other articles:
|
|
 |
|
  M.S. Theas, C. Rival, S. Jarazo-Dietrich, P. Jacobo, V.A. Guazzone, and L. Lustig Tumour necrosis factor-{alpha} released by testicular macrophages induces apoptosis of germ cells in autoimmune orchitis Hum. Reprod., August 1, 2008; 23(8): 1865 - 1872. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. A Tarulli, S. J Meachem, S. Schlatt, and P. G Stanton Regulation of testicular tight junctions by gonadotrophins in the adult Djungarian hamster in vivo Reproduction, June 1, 2008; 135(6): 867 - 877. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. H. N. Yan, D. D. Mruk, W. M. Lee, and C. Y. Cheng Blood-testis barrier dynamics are regulated by testosterone and cytokines via their differential effects on the kinetics of protein endocytosis and recycling in Sertoli cells FASEB J, June 1, 2008; 22(6): 1945 - 1959. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. D. Mruk, B. Silvestrini, and C. Y. Cheng Anchoring Junctions As Drug Targets: Role in Contraceptive Development Pharmacol. Rev., June 1, 2008; 60(2): 146 - 180. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Sarkar, P. P. Mathur, C. Y. Cheng, and D. D. Mruk Interleukin 1 Alpha (IL1A) Is a Novel Regulator of the Blood-Testis Barrier in the Rat Biol Reprod, March 1, 2008; 78(3): 445 - 454. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Q.F. Wang and C. Y. Cheng A seamless trespass: germ cell migration across the seminiferous epithelium during spermatogenesis J. Cell Biol., August 9, 2007; 178(4): 549 - 556. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Xia, D. D. Mruk, and C. Y. Cheng C-type natriuretic peptide regulates blood-testis barrier dynamics in adult rat testes PNAS, March 6, 2007; 104(10): 3841 - 3846. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. P Y Lie, W. Xia, C. Q F Wang, D. D Mruk, H. H N Yan, C.-h. Wong, W. M Lee, and C Y. Cheng Dynamin II interacts with the cadherin- and occludin-based protein complexes at the blood-testis barrier in adult rat testes J. Endocrinol., December 1, 2006; 191(3): 571 - 586. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | CONTACT US | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
