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Journal of Endocrinology (2006) 190, 213-223       DOI: 10.1677/joe.1.06636
© 2006 Society for Endocrinology
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Centrally administered neuropeptide W-30 activates magnocellular neurosecretory cells in the supraoptic and paraventricular nuclei with neurosecretion in rats

Makoto Kawasaki1,2, Tatsushi Onaka3, Masamitsu Nakazato4, Jun Saito1,2, Takashi Mera1, Hirofumi Hashimoto1, Hiroaki Fujihara1, Nobukazu Okimoto2, Hideo Ohnishi2, Toshitaka Nakamura2 and Yoichi Ueta1

1 Departments of Physiology and
2 Orthopaedics, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan
3 Department of Physiology, Jichi Medical School, Tochigi 329-0498, Japan
4 Department of Third Internal Medicine, Miyazaki University, Miyazaki 889-1692, Japan

(Requests for offprints should be addressed to Y Ueta; Email: yoichi{at}med.uoeh-u.ac.jp)

We examined the effects of i.c.v. administration of neuro-peptide W-30 (NPW30) on plasma arginine vasopressin (AVP) and plasma oxytocin (OXT) using RIA. The induction of c-fos mRNA, AVP heteronuclear (hn)RNA, and c-Fos protein (Fos) in the supraoptic nucleus (SON) and paraventricular nucleus (PVN) of rats were also investigated using in situ hybridization histochemistry for c-fos mRNA and AVP hnRNA, and immunohistochemistry for Fos. Both plasma AVP and OXT were significantly increased at 5 and 15 min after i.c.v. administration of NPW30 (2.8 nmol/rat). In situ hybridization histochemistry revealed that the induction of c-fos mRNA and AVP hnRNA in the SON and PVN were significantly increased 15, 30, and 60 min after i.c.v. administration of NPW30 (1.4 nmol/rat). Dual immunostaining for Fos/AVP and Fos/OXT revealed that both AVP-like immunoreactive (LI) cells and OXT-LI cells exhibited nuclear Fos-LI in the SON and PVN, 90 min after i.c.v. administration of NPW30 (2.8 nmol/rat). These results suggest that central NPW30 may be involved in the regulation of secretion of AVP and OXT in the magnocellular neurosecretory cells in the SON and PVN.






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