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¹ Bone Biology Group, Division of Gene Function and Development, Roslin Institute, Edinburgh EH25 9PS, UK
² Bone and Endocrine Research Group, Royal Hospital for Sick Children, Glasgow G3 8SJ, UK

(Requests for offprints should be addressed to V E MacRae; vicky.macrae{at}bbsrc.ac.uk)

Childhood chronic inflammatory disease can be associated with transient and permanent growth retardation. This study examined the potential for spontaneous growth recovery following pro-inflammatory cytokine exposure. Murine ATDC5 chondrogenic cells and postnatal metatarsals were exposed to interleukin (IL)-1ß, IL-6 and tumour necrosis factor- (TNF), and their growth and proliferative capacity were determined following recovery. TNF and IL-1ß reduced chondrocyte proliferation and aggrecan and collagen types II and X expression at minimum concentrations of 10 ng/ml and 0.1 ng/ml respectively. TNF but not IL-1ß exposure led to increased caspase-3 activity and altered cellular morphology, consistent with reduced viability. Cytokine exposure particularly inhibited proteoglycan synthesis. This effect was dose and duration dependent. Compared with the control, IL-1ß and TNF led to a 71% and 45% reduction in metatarsal growth after 8 days of exposure respectively (P < 0.05). An additive effect of IL-1ß combined with TNF was observed (110% decrease; P < 0.05). Metatarsals exposed to IL-1ß or TNF individually for a 2-day period, and allowed to recover spontaneously in the absence of cytokines for a further 6 days, showed normal growth trajectories. In combination, growth was 59% lower (P < 0.01) compared with control metatarsals at the end of the recovery period. Exposure to the combination for 4 days followed by a 4-day recovery period resulted in 87% decrement compared with controls (P < 0.05). IL-6 did not alter any parameter studied. IL-1ß and TNF exert diverse inhibitory effects on ATDC5 chondrocyte dynamics and metatarsal growth. The extent of recovery following cytokine exposure depends on the duration of exposure, and may be incomplete following longer periods of exposure.

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