JOE
HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


Journal of Endocrinology (2005) 184, 257-266       DOI: 10.1677/joe.1.05926
© 2005 Society for Endocrinology
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (24)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yin, H
Right arrow Articles by Tsutsui, K
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yin, H
Right arrow Articles by Tsutsui, K

A novel G protein-coupled receptor for gonadotropin-inhibitory hormone in the Japanese quail (Coturnix japonica): identification, expression and binding activity

H Yin1,2, K Ukena1,2, T Ubuka1,2 and K Tsutsui1,2

1 Laboratory of Brain Science, Faculty of Integrated Arts and Sciences, Hiroshima University, Higashi-Hiroshima 739-8521, Japan
2 Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation, Tokyo 150-0002, Japan

(Requests for offprints should be addressed to K Tsutsui; Email: tsutsui{at}hiroshima-u.ac.jp)

We recently identified a novel hypothalamic dodecapeptide inhibiting gonadotropin release in the Japanese quail (Coturnix japonica). This novel peptide was therefore named gonadotropin-inhibitory hormone (GnIH). The GnIH precursor encoded one GnIH and two GnIH-related peptides (GnIH-RP-1 and GnIH-RP-2) that shared the same C-terminal motif, Leu-Pro-Xaa-Arg-Phe-NH2 (Xaa=Leu or Gln; LPXRF-amide peptides). Identification of the receptor for GnIH is crucial to elucidate the mode of action of GnIH. We therefore identified the receptor for GnIH in the quail diencephalon and characterized its expression and binding activity. We first cloned a cDNA encoding a putative GnIH receptor by a combination of 3' and 5' rapid amplification of cDNA ends (RACE) using PCR primers designed from the sequence for the receptor for rat RF-amide-related peptide (RFRP), an orthologous peptide of GnIH. Hydrophobic analysis revealed that the putative GnIH receptor possessed seven transmembrane domains, indicating a new member of the G protein-coupled receptor superfamily. The crude membrane fraction of COS-7 cells transfected with the putative GnIH receptor cDNA specifically bound to GnIH and GnIH-RPs in a concentration-dependent manner. Scatchard plot analysis of the binding showed that the identified GnIH receptor possessed a single class of high-affinity binding sites (Kd=0.752 nM, Bmax=24.8 fmol/mg protein). Southern blotting analysis of reverse transcriptase-mediated PCR products revealed the expression of GnIH receptor mRNA in the pituitary gland and several brain regions including diencephalon in the quail. These results suggest that GnIH acts directly on the pituitary via GnIH receptor to inhibit gonadotropin release. GnIH may also act on the hypothalamus to inhibit gonadotropin-releasing hormone release.




This article has been cited by other articles:


Home page
EndocrinologyHome page
S. Zhao, E. Zhu, C. Yang, G. E. Bentley, K. Tsutsui, and L. J. Kriegsfeld
RFamide-Related Peptide and Messenger Ribonucleic Acid Expression in Mammalian Testis: Association with the Spermatogenic Cycle
Endocrinology, February 1, 2010; 151(2): 617 - 627.
[Abstract] [Full Text] [PDF]


Home page
EndocrinologyHome page
V. S. Chowdhury, K. Yamamoto, T. Ubuka, G. E. Bentley, A. Hattori, and K. Tsutsui
Melatonin Stimulates the Release of Gonadotropin-Inhibitory Hormone by the Avian Hypothalamus
Endocrinology, January 1, 2010; 151(1): 271 - 280.
[Abstract] [Full Text] [PDF]


Home page
EndocrinologyHome page
I. P. Sari, A. Rao, J. T. Smith, A. J. Tilbrook, and I. J. Clarke
Effect of RF-Amide-Related Peptide-3 on Luteinizing Hormone and Follicle-Stimulating Hormone Synthesis and Secretion in Ovine Pituitary Gonadotropes
Endocrinology, December 1, 2009; 150(12): 5549 - 5556.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
E. D. Kirby, A. C. Geraghty, T. Ubuka, G. E. Bentley, and D. Kaufer
Stress increases putative gonadotropin inhibitory hormone and decreases luteinizing hormone in male rats
PNAS, July 7, 2009; 106(27): 11324 - 11329.
[Abstract] [Full Text] [PDF]


Home page
EndocrinologyHome page
E. Ducret, G. M. Anderson, and A. E. Herbison
RFamide-Related Peptide-3, a Mammalian Gonadotropin-Inhibitory Hormone Ortholog, Regulates Gonadotropin-Releasing Hormone Neuron Firing in the Mouse
Endocrinology, June 1, 2009; 150(6): 2799 - 2804.
[Abstract] [Full Text] [PDF]


Home page
EndocrinologyHome page
G. M. Anderson, H.-L. Relf, M. Z. Rizwan, and J. J. Evans
Central and Peripheral Effects of RFamide-Related Peptide-3 on Luteinizing Hormone and Prolactin Secretion in Rats
Endocrinology, April 1, 2009; 150(4): 1834 - 1840.
[Abstract] [Full Text] [PDF]


Home page
EndocrinologyHome page
M. Z. Rizwan, R. Porteous, A. E. Herbison, and G. M. Anderson
Cells Expressing RFamide-Related Peptide-1/3, the Mammalian Gonadotropin-Inhibitory Hormone Orthologs, Are Not Hypophysiotropic Neuroendocrine Neurons in the Rat
Endocrinology, March 1, 2009; 150(3): 1413 - 1420.
[Abstract] [Full Text] [PDF]


Home page
Integr. Comp. Biol.Home page
T. Ubuka, N. L. McGuire, R. M. Calisi, N. Perfito, and G. E. Bentley
The control of reproductive physiology and behavior by gonadotropin-inhibitory hormone
Integr. Comp. Biol., November 1, 2008; 48(5): 560 - 569.
[Abstract] [Full Text] [PDF]


Home page
EndocrinologyHome page
I. J. Clarke, I. P. Sari, Y. Qi, J. T. Smith, H. C. Parkington, T. Ubuka, J. Iqbal, Q. Li, A. Tilbrook, K. Morgan, et al.
Potent Action of RFamide-Related Peptide-3 on Pituitary Gonadotropes Indicative of a Hypophysiotropic Role in the Negative Regulation of Gonadotropin Secretion
Endocrinology, November 1, 2008; 149(11): 5811 - 5821.
[Abstract] [Full Text] [PDF]


Home page
J EndocrinolHome page
M. Murakami, T. Matsuzaki, T. Iwasa, T. Yasui, M. Irahara, T. Osugi, and K. Tsutsui
Hypophysiotropic role of RFamide-related peptide-3 in the inhibition of LH secretion in female rats
J. Endocrinol., October 1, 2008; 199(1): 105 - 112.
[Abstract] [Full Text] [PDF]


Home page
Proc R Soc BHome page
T. J Greives, L. J Kriegsfeld, G. E Bentley, K. Tsutsui, and G. E Demas
Recent advances in reproductive neuroendocrinology: a role for RFamide peptides in seasonal reproduction?
Proc R Soc B, September 7, 2008; 275(1646): 1943 - 1951.
[Abstract] [Full Text] [PDF]


Home page
EndocrinologyHome page
V. S. Chowdhury, K. Yamamoto, I. Saeki, I. Hasunuma, T. Shimura, and K. Tsutsui
Melatonin Stimulates the Release of Growth Hormone and Prolactin by a Possible Induction of the Expression of Frog Growth Hormone-Releasing Peptide and Its Related Peptide-2 in the Amphibian Hypothalamus
Endocrinology, March 1, 2008; 149(3): 962 - 970.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
K. Tsutsui, K. Inoue, H. Miyabara, S. Suzuki, Y. Ogura, and S. Haraguchi
7{alpha}-Hydroxypregnenolone Mediates Melatonin Action Underlying Diurnal Locomotor Rhythms
J. Neurosci., February 27, 2008; 28(9): 2158 - 2167.
[Abstract] [Full Text] [PDF]


Home page
ReproductionHome page
S. R Maddineni, O. M Ocon-Grove, S. M Krzysik-Walker, G. L Hendricks III, and R. Ramachandran
Gonadotropin-inhibitory hormone (GnIH) receptor gene is expressed in the chicken ovary: potential role of GnIH in follicular maturation
Reproduction, February 1, 2008; 135(2): 267 - 274.
[Abstract] [Full Text] [PDF]


Home page
EndocrinologyHome page
T. Ubuka, S. Kim, Y.-c. Huang, J. Reid, J. Jiang, T. Osugi, V. S. Chowdhury, K. Tsutsui, and G. E. Bentley
Gonadotropin-Inhibitory Hormone Neurons Interact Directly with Gonadotropin-Releasing Hormone-I and -II Neurons in European Starling Brain
Endocrinology, January 1, 2008; 149(1): 268 - 278.
[Abstract] [Full Text] [PDF]


Home page
Poult. Sci.Home page
J. B. Zawilska, A. Lorenc, M. Berezinska, B. Vivien-Roels, P. Pevet, and D. J. Skene
Photoperiod-Dependent Changes in Melatonin Synthesis in the Turkey Pineal Gland and Retina
Poult. Sci., July 1, 2007; 86(7): 1397 - 1405.
[Abstract] [Full Text] [PDF]


Home page
EndocrinologyHome page
T. Ubuka, K. Ukena, P. J. Sharp, G. E. Bentley, and K. Tsutsui
Gonadotropin-Inhibitory Hormone Inhibits Gonadal Development and Maintenance by Decreasing Gonadotropin Synthesis and Release in Male Quail
Endocrinology, March 1, 2006; 147(3): 1187 - 1194.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
L. J. Kriegsfeld, D. F. Mei, G. E. Bentley, T. Ubuka, A. O. Mason, K. Inoue, K. Ukena, K. Tsutsui, and R. Silver
Identification and characterization of a gonadotropin-inhibitory system in the brains of mammals
PNAS, February 14, 2006; 103(7): 2410 - 2415.
[Abstract] [Full Text] [PDF]




HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2005 by the Society for Endocrinology.