HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) An erratum has been published Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Web of Science (5) Citing Articles via Google Scholar Google Scholar Articles by Berteaux, N Articles by Curgy, J-J Search for Related Content PubMed PubMed Citation Articles by Berteaux, N Articles by Curgy, J-J

Unité INSERM ESPRI: Signalisation des facteurs de croissance dans le cancer du sein, Protéomique fonctionnelle, Université des Sciences et Technologies de Lille, 59655 Villeneuve d’Ascq cedex, France
¹ Laboratoire de Physiologie cellulaire, INSERM EPI-9938, Université des Sciences et Technologies de Lille, 59655 Villeneuve d’Ascq, France
² Laboratoire d’Anatomie et Cytologie pathologiques, Hôpital Huriez, Pôle Recherche, Faculté de Médecine, 1 Place de Verdun, Centre Hospitalier Universitaire, 59045 Lille cedex, France
³ Immunopathologie cellulaire des maladies infectieuses, CNRS UMR 8527, Institut de Biologie Moléculaire, Institut Pasteur, 1 Rue Calmette, 59019 Lille cedex, France
⁴ Faculté des Sciences Jean Perrin, Université d’Artois, 62307 Lens cedex, France

(Requests for offprints should be addressed to J-J Curgy; Email curgy{at}univ-lille1.fr)

The H19 gene is transcribed in an mRNA-like noncoding RNA. When tumors of various organs or cell types are considered, H19 oncogene or tumor-suppressor status remains controversial. To address the potential regulation of H19 gene expression by an androgen steroid hormone (DHT: dihydrotestosterone) or by a peptidic hormone (PRL: prolactin), we performed experiments in rats systemically treated with chemical mediators. This range of in vivo experiments demonstrated that chronic hyperprol-actinemia upregulated the H19 expression in epithelial and stromal cells whereas DHT downregulated the gene. PRL and DHT appeared to be opposite mediators in the H19 RNA synthesis. We investigated these hormonal effects in three human prostate epithelial cell lines. In LNCaP cancer cells, the opposite effect of PRL and DHT was corroborated. However, in normal cells (PNT1A), H19 remained insensitive to the hormones in fetal calf serum (FCS) medium but became responsive in a serum-stripped medium. In the DU-145 cancer cell line, tested for its androgen-independence and aggressiveness, the hormones had no effect on H19 expression whatever the culture conditions. Finally, we demonstrated that PRL upregulated the H19 expression in LNCaP cells by the JAK2–STAT5 transduction pathway. We conclude that H19 expression is regulated by both a peptidic and a male steroid hormone.