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¹ Department of Agricultural Sciences, Imperial College London, Wye Campus, Ashford, Kent TN25 5AH, UK
² Centre for Reproduction and Early Life, Institute of Clinical Sciences, University Hospital, Nottingham NG7 2UH, UK
³ Faculté de Médecine Necker-Enfants-Malades, CNRS-UPR 9078 (7ème étage), 156, rue de Vaugirard, 75730 Paris, CEDEX 15, France

(Requests for offprints should be addressed to Alison Mostyn, Academic Division of Child Health, School of Human Development, University Hospital, Nottingham NG7 2UH, UK; Email: alison.mostyn{at}nottingham.ac.uk)

The present study aimed to determine whether porcine genotype and/or postnatal age influenced mRNA abundance or protein expression of uncoupling protein (UCP)2 or 3 in subcutaneous adipose tissue (AT) and skeletal muscle (SM) and the extent to which these differences are associated with breed-specific discordance in endocrine and metabolic profiles. Piglets from commercial and Meishan litters were ranked according to birth weight. Tissue samples were obtained from the three median piglets from each litter on either day 0, 4, 7, 14 or 21 of neonatal life. UCP2 protein abundance in AT was similar between genotypes on the first day of life, but it was elevated at all subsequent postnatal ages (P<0.05) in AT of Meishan piglets. In contrast, UCP2 mRNA abundance was lower in Meishans up to 14 days of age. UCP2 mRNA expression was not correlated with protein abundance in either breed at any age. UCP3 mRNA in AT was similar between breeds up to day 7; thereafter, expression was higher (general linear model, P<0.05) in Meishan piglets. Conversely, UCP3 mRNA expression in SM was higher in commercial piglets after day 7. Colonic temperature remained lower in Meishan than commercial piglets throughout the study; this was most obvious in the immediate post-partum period when Meishan piglets had lower (P<0.05) plasma triiodothyronine. In conclusion, we have demonstrated that porcine genotype influences the expression and abundance of UCP2 and 3, an influence which may, in part, be due to the distinctive endocrine profiles associated with each genotype.

This article has been cited by other articles:

				A. Mostyn, S. Sebert, J. C Litten, K. S Perkins, J. Laws, M. E Symonds, and L. Clarke Influence of porcine genotype on the abundance of thyroid hormones and leptin in sow milk and its impact on growth, metabolism and expression of key adipose tissue genes in offspring. J. Endocrinol., September 1, 2006; 190(3): 631 - 639. [Abstract] [Full Text] [PDF]

				M G Gnanalingham, A Mostyn, D S Gardner, T Stephenson, and M E Symonds Developmental regulation of the lung in preparation for life after birth: hormonal and nutritional manipulation of local glucocorticoid action and uncoupling protein-2. J. Endocrinol., March 1, 2006; 188(3): 375 - 386. [Abstract] [Full Text] [PDF]

				A. Mostyn, J. C. Litten, K. S. Perkins, P. J. Euden, A. M. Corson, M. E. Symonds, and L. Clarke Influence of size at birth on the endocrine profiles and expression of uncoupling proteins in subcutaneous adipose tissue, lung, and muscle of neonatal pigs Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2005; 288(6): R1536 - R1542. [Abstract] [Full Text] [PDF]