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Castrated hypophysectomized male mice of the CHI strain showed a small response in mammary duct growth to injections of oestrone and progesterone. A greater degree of duct growth resulted from the use of growth hormone (GH) in addition to these steroids, and formation of alveoli in addition to duct growth followed treatment with the steroids and prolactin. A greater degree of mammary development, with duct growth and the presence of many alveoli, was seen in mice treated with the steroids and both prolactin and GH.

In contrast to the females of this strain, which normally produce ten mammary glands, the males had variable numbers, ranging from none to five.

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