| HOME | HELP | CONTACT US | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
We have recently provided evidence for the desensitization of oxytocin receptors in human myometrial cells. In the present study, we have investigated the possible mechanisms by which oxytocin (OT) might regulate OT receptor density. The steady state level of OT binding in cultured myometrial cells was 220 x 103 binding sites/cell, but this was time-dependently reduced to 27 x 103 sites/cell by exposure to OT for up to 20 h. Similarly, OT exposure decreased the binding of OT to cell membranes. In contrast, Western blotting data showed that the total amount of OT receptor protein was not affected by OT treatment for up to 48 h. Flow cytometry experiments demonstrated that OT receptors are not internalized during prolonged exposure of the cells to OT. However, RNase protection assays and Northern analysis showed that OT receptor mRNA was reduced by OT treatment to reach a new low steady state level with a time course similar to that of the disappearance of cell surface OT binding sites. Possible mechanisms involved in mRNA down-regulation include transcriptional suppression and destabilization of mRNA by RNA binding proteins.
Journal of Endocrinology (1997) 154, 7–18
This article has been cited by other articles:
|
|
 |
|
  A. J. Barrett, R. D. Murray, R. M. Christley, H. Dobson, and R. F. Smith Effects of the administration of oxytocin or carbetocin to dairy cows at parturition on their subsequent fertility Vet Rec., November 21, 2009; 165(21): 623 - 626. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A J Tyson-Capper, E A Shiells, and S C Robson Interplay between polypyrimidine tract binding protein-associated splicing factor and human myometrial progesterone receptors J. Mol. Endocrinol., July 1, 2009; 43(1): 29 - 41. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. K. R. S. Magalhaes, J. C. A. Carvalho, R. K. Parkes, J. Kingdom, Yong Li, and M. Balki Oxytocin Pretreatment Decreases Oxytocin-induced Myometrial Contractions in Pregnant Rats in a Concentration-dependent But Not Time-dependent Manner Reproductive Sciences, May 1, 2009; 16(5): 501 - 508. [Abstract] [PDF]
|
|
|
|
 |
|
 P. Ciarmela, E. Wiater, and W. Vale Activin-A in Myometrium: Characterization of the Actions on Myometrial Cells Endocrinology, May 1, 2008; 149(5): 2506 - 2516. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Ball, J. W. Wang, S. Wong, B. Zielnik, J. Mitchell, N. Wang, M. B. Stemerman, and B. F. Mitchell Phorbol ester treatment of human myometrial cells suppresses expression of oxytocin receptor through a mechanism that does not involve activator protein-1 Am J Physiol Endocrinol Metab, November 1, 2006; 291(5): E922 - E928. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. P. Smith, V. J. Ayad, S. J. Mundell, C. A. McArdle, E. Kelly, and A. Lopez Bernal Internalization and Desensitization of the Oxytocin Receptor Is Inhibited by Dynamin and Clathrin Mutants in Human Embryonic Kidney 293 Cells Mol. Endocrinol., February 1, 2006; 20(2): 379 - 388. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O.N. Richter, C. Bartz, J. Dowaji, M. Kupka, J. Reinsberg, U. Ulrich, and W. Rath Contractile reactivity of human myometrium in isolated non-pregnant uteri Hum. Reprod., January 1, 2006; 21(1): 36 - 45. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Hasbi, D. Devost, S. A. Laporte, and H. H. Zingg Real-Time Detection of Interactions between the Human Oxytocin Receptor and G Protein-Coupled Receptor Kinase-2 Mol. Endocrinol., May 1, 2004; 18(5): 1277 - 1286. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O.N. Richter, K. Kubler, J. Schmolling, M. Kupka, J. Reinsberg, U. Ulrich, H. van derVen, E. Wardelmann, and K. van derVen Oxytocin receptor gene expression of estrogen-stimulated human myometrium in extracorporeally perfused non-pregnant uteri Mol. Hum. Reprod., May 1, 2004; 10(5): 339 - 346. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. H. Burbach, S. M. Luckman, D. Murphy, and H. Gainer Gene Regulation in the Magnocellular Hypothalamo-Neurohypophysial System Physiol Rev, July 1, 2001; 81(3): 1197 - 1267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Schmid, S. Wong, and B. F. Mitchell Transcriptional Regulation of Oxytocin Receptor by Interleukin-1{beta} and Interleukin-6 Endocrinology, April 1, 2001; 142(4): 1380 - 1385. [Abstract] [Full Text]
|
|
|
|
|
|
G. Gimpl and F. Fahrenholz The Oxytocin Receptor System: Structure, Function, and Regulation Physiol Rev, April 1, 2001; 81(2): 629 - 683. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Imamura, C. E. Luedke, S. K. Vogt, and L. J. Muglia Oxytocin modulates the onset of murine parturition by competing ovarian and uterine effects Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2000; 279(3): R1061 - R1067. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. N. Rauk and J.-P. Chiao Oxytocin Signaling in Human Myometrium Is Impaired by Prolonged Exposure to Interleukin-1 Biol Reprod, September 1, 2000; 63(3): 846 - 850. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. J. Pollard, C. Sparey, S. C. Robson, A. R. Krainer, and G. N. Europe-Finner Spatio-Temporal Expression of the Trans-Acting Splicing Factors SF2/ASF and Heterogeneous Ribonuclear Proteins A1/A1B in the Myometrium of the Pregnant Human Uterus: A Molecular Mechanism for Regulating Regional Protein Isoform Expression in Vivo J. Clin. Endocrinol. Metab., May 1, 2000; 85(5): 1928 - 1936. [Abstract] [Full Text]
|
|
|
|
|
|
T. Engstrom, P. Bratholm, H. Vilhardt, and N. J. Christensen Effect of Oxytocin Receptor and ß2-Adrenoceptor Blockade on Myometrial Oxytocin Receptors in Parturient Rats Biol Reprod, February 1, 1999; 60(2): 322 - 329. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Hatthachote, J. Morgan, W. Dunlop, G. N. Europe-Finner, and J. I. Gillespie Gestational Changes in the Levels of Transforming Growth Factor-{beta}1 (TGF{beta}1) and TGF{beta} Receptor Types I and II in the Human Myometrium J. Clin. Endocrinol. Metab., August 1, 1998; 83(8): 2987 - 2992. [Abstract] [Full Text]
|
|
| HOME | HELP | CONTACT US | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
