| HOME | HELP | CONTACT US | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The aim of this study was to examine the expression and regulation of type 1 17β-hydroxysteroid dehydrogenase (type 1 17-HSD) enzyme protein and mRNA, and 17-HSD activity in human granulosa cells. The cells were obtained from patients taking part in an in vitro fertilization programme. The cells from each patient were divided into two groups: cells obtained from preovulatory follicles (LGC=granulosa cells from large follicles 
18 mm in diameter), and cells from other visible follicles (SGC=granulosa cells from small follicles, less than 15 mm in diameter). The identity of 17-HSD enzyme protein expressed in human granulosa cells with placental cytosolic 17-HSD (type 1 17-HSD) was assessed by immunoblot analysis using polyclonal antibodies, and the enzyme was immunolocalized in the cytoplasm of granulosa cells. Type 1 17-HSD protein concentration, 17-HSD and cytochrome P450 aromatase (P450arom) activities and oestradiol (OE2) production in cells from LGC were significantly lower than the corresponding values obtained in SGC in the same patient (paired t-test). The type 1 17-HSD protein concentration, 17-HSD activity and P450arom activity were 140±16% (mean±S.E.M.), 121±22% and 113±26% higher in cells from SGC, which was also reflected in a 70±12% higher OE2 production in these cells. In freshly isolated cells from LGC or SGC, a high correlation between 17-HSD and P450arom activities was observed (r=0·93, P<0·001). In long-term cultured cells, type 1 17-HSD was stably expressed at least until day 9, while P450arom expression decreased. In addition, treatments with gonadotrophins did not affect type 1 17-HSD protein concentration and 17-HSD activity. In contrast to this, both P450arom activity and OE2 production were significantly increased (P<0·05). The data, therefore, suggest that type 1 17-HSD and P450arom are expressed in parallel during the latest stages of follicular maturation but, in cultured granulosa-luteal cells, the enzymes are regulated by distinct mechanisms.
Journal of Endocrinology (1994) 143, 139–150
This article has been cited by other articles:
|
|
 |
|
  P. Rantakari, L. Strauss, R. Kiviranta, H. Lagerbohm, J. Paviala, I. Holopainen, S. Vainio, P. Pakarinen, and M. Poutanen Placenta Defects and Embryonic Lethality Resulting from Disruption of Mouse Hydroxysteroid (17-{beta}) Dehydrogenase 2 Gene Mol. Endocrinol., March 1, 2008; 22(3): 665 - 675. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Saloniemi, T. Lamminen, K. Huhtinen, M. Welsh, P. Saunders, H. Kujari, and M. Poutanen Activation of Androgens by Hydroxysteroid (17{beta}) Dehydrogenase 1 in Vivo as a Cause of Prenatal Masculinization and Ovarian Benign Serous Cystadenomas Mol. Endocrinol., November 1, 2007; 21(11): 2627 - 2636. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. E. Vaskivuo, M. Maentausta, S. Torn, O. Oduwole, A. Lonnberg, R. Herva, V. Isomaa, and J. S. Tapanainen Estrogen Receptors and Estrogen-Metabolizing Enzymes in Human Ovaries during Fetal Development J. Clin. Endocrinol. Metab., June 1, 2005; 90(6): 3752 - 3756. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. L. Britt, P. G. Stanton, M. Misso, E. R. Simpson, and J. K. Findlay The Effects of Estrogen on the Expression of Genes Underlying the Differentiation of Somatic Cells in the Murine Gonad Endocrinology, August 1, 2004; 145(8): 3950 - 3960. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Husen, J. Adamski, A. Bruns, D. Deluca, K. Fuhrmann, G. Moller, I. Schwabe, and A. Einspanier Characterization of 17{beta}-Hydroxysteroid Dehydrogenase Type 7 in Reproductive Tissues of the Marmoset Monkey Biol Reprod, June 1, 2003; 68(6): 2092 - 2099. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.A. Whitehead and M. Lacey Phytoestrogens inhibit aromatase but not 17{beta}-hydroxysteroid dehydrogenase (HSD) type 1 in human granulosa-luteal cells: evidence for FSH induction of 17{beta}-HSD Hum. Reprod., March 1, 2003; 18(3): 487 - 494. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F.M. Morán, A.J. Conley, C.J. Corbin, E. Enan, C. VandeVoort, J.W. Overstreet, and B.L. Lasley 2,3,7,8-Tetrachlorodibenzo-p-Dioxin Decreases Estradiol Production Without Altering the Enzyme Activity of Cytochrome P450 Aromatase of Human Luteinized Granulosa Cells In Vitro Biol Reprod, April 1, 2000; 62(4): 1102 - 1108. [Abstract] [Full Text]
|
|
|
|
|
|
S. Leivonen, Y.-s. Piao, H. Peltoketo, P. Numchaisrika, R. Vihko, and P. Vihko Identification of Essential Subelements in the hHSD17B1 Enhancer: Difference in Function of the Enhancer and That of the hHSD17BP1 Analog Is due to -480C and -486G Endocrinology, August 1, 1999; 140(8): 3478 - 3487. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. W. Sawicki, M. Erman, T. Puranen, P. Vihko, and D. Ghosh Structure of the ternary complex of human 17beta -hydroxysteroid dehydrogenase type 1 with 3-hydroxyestra-1,3,5,7-tetraen-17-one (equilin) and NADP+ PNAS, February 2, 1999; 96(3): 840 - 845. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Nokelainen, H. Peltoketo, R. Vihko, and P. Vihko Expression Cloning of a Novel Estrogenic Mouse 17{beta}-Hydroxysteroid Dehydrogenase/ 17-Ketosteroid Reductase (m17HSD7), Previously Described as a Prolactin Receptor-Associated Protein (PRAP) in Rat Mol. Endocrinol., July 1, 1998; 12(7): 1048 - 1059. [Abstract] [Full Text]
|
|
|
|
|
|
Y.-s. Piao, H. Peltoketo, P. Vihko, and R. Vihko The Proximal Promoter Region of the Gene Encoding Human 17{beta}-Hydroxysteroid Dehydrogenase Type 1 Contains GATA, AP-2, and Sp1 Response Elements: Analysis of Promoter Function in Choriocarcinoma Cells Endocrinology, August 1, 1997; 138(8): 3417 - 3425. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Puranen, M. Poutanen, D. Ghosh, R. Vihko, and P. Vihko Origin of Substrate Specificity of Human and Rat 17{beta}-Hydroxysteroid Dehydrogenase Type 1, Using Chimeric Enzymes and Site-Directed Substitutions Endocrinology, August 1, 1997; 138(8): 3532 - 3539. [Abstract] [Full Text]
|
|
|
|
|
|
L. A. Akinola, M. Poutanen, R. Vihko, and P. Vihko Expression of 17{beta}-Hydroxysteroid Dehydrogenase Type 1 and Type 2, P450 Aromatase, and 20{alpha}-Hydroxysteroid Dehydrogenase Enzymes in Immature, Mature, and Pregnant Rats Endocrinology, July 1, 1997; 138(7): 2886 - 2892. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-s. Piao, H. Peltoketo, A. Jouppila, and R. Vihko Retinoic Acids Increase 17{beta}-Hydroxysteroid Dehydrogenase Type 1 Expression in JEG-3 and T47D Cells, but the Stimulation Is Potentiated by Epidermal Growth Factor, 12-O-Tetradecanoylphorbol-13-Acetate, and Cyclic Adenosine 3',5'-Monophosphate Only in JEG-3 Cells Endocrinology, March 1, 1997; 138(3): 898 - 904. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Puranen, M. Poutanen, D. Ghosh, P. Vihko, and R. Vihko Characterization of Structural and Functional Properties of Human 17{beta}-Hydroxysteroid Dehydrogenase Type 1 Using Recombinant Enzymes and Site-Directed Mutagenesis Mol. Endocrinol., January 1, 1997; 11(1): 77 - 86. [Abstract] [Full Text]
|
|
| HOME | HELP | CONTACT US | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
