HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Campbell, B K Articles by Scaramuzzi, R J Search for Related Content PubMed PubMed Citation Articles by Campbell, B K Articles by Scaramuzzi, R J

Transforming growth factor (TGF) inhibits hormone production by cultured follicular cells but evidence of an effect of TGF on ovarian hormone secretion in vivo is still required. Eleven ewes with an autotransplanted ovary received, by ovarian arterial infusion, either 5 µg/h recombinant rat TGF (n=6) or placebo (n=5) for 12 h on day 10 of the luteal phase. Two hours before the start and 1 hour before the end of the infusion each ewe received a single injection of gonadotrophin-releasing hormone (GnRH; 150 ng i.v.). Two hours after the end of the infusion luteal regression was induced with prostaglandin F₂ (PGF₂; 125 µg i.m.). Ovarian and jugular venous blood samples were taken at 10-min, 15-min or 4-h intervals from 2 h before the start of the infusion until 96 h after PGF₂ and the rates of secretion of ovarian oestradiol, inhibin, progesterone and androstenedione were determined. Jugular venous concentrations of LH and FSH were also measured and follicle populations monitored by real-time ultrasound scanning.

Infusion of TGF resulted in a significant (P<0.05) depression in the amplitude of the pulsatile response of oestradiol and androstenedione secretion to the GnRH-induced LH pulse at the end of the infusion. Ovarian inhibin secretion was acutely suppressed by TGF infusion (P<0·001) and remained lower than controls for the period of the experiment. Luteal phase progesterone secretion was also acutely inhibited (P<0·001) by infusion of TGF and in one treated ewe progesterone secretion was elevated 48–84 h after PGF₂. Jugular venous concentrations of FSH in TGF-treated ewes were significantly (P<0·001) elevated over controls during the first 48 h of the follicular phase and the LH surge was delayed for about 10 h (P<0·05). Infusion of TGF caused a marked decline (P<0·05) in the number of large follicles within 12 h of the end of the infusion. Two of the six treated ewes, including the one with high follicular phase progesterone, had unusually large (8·7 and 10 mm) follicles present from 48–96 h after PGF₂.

We conclude that direct arterial infusion of TGF results in acute inhibition of ovarian steroid and inhibin secretion that is associated with induction of atresia in the population of large follicles. The lack of feedback of ovarian hormones results in a rebound increase of FSH which stimulates the growth of more ovarian follicles and the eventual re-establishment of ovarian hormone secretion and normal cyclicity.

Journal of Endocrinology (1994) 143, 13–24

This article has been cited by other articles:

				B.K. Campbell, N.R. Kendall, and D.T. Baird Effect of Direct Ovarian Infusion of Bone Morphogenetic Protein 6 (BMP6) on Ovarian Function in Sheep Biol Reprod, November 1, 2009; 81(5): 1016 - 1023. [Abstract] [Full Text] [PDF]

				D. C. Woods and A. L. Johnson Phosphatase Activation by Epidermal Growth Factor Family Ligands Regulates Extracellular Regulated Kinase Signaling in Undifferentiated Hen Granulosa Cells Endocrinology, October 1, 2006; 147(10): 4931 - 4940. [Abstract] [Full Text] [PDF]

				M Munoz-Gutierrez, P A Findlay, C L Adam, G Wax, B K Campbell, N R Kendall, M Khalid, M Forsberg, and R J Scaramuzzi The ovarian expression of mRNAs for aromatase, IGF-I receptor, IGF-binding protein-2, -4 and -5, leptin and leptin receptor in cycling ewes after three days of leptin infusion Reproduction, December 1, 2005; 130(6): 869 - 881. [Abstract] [Full Text] [PDF]

				N R Kendall, C G Gutierrez, R J Scaramuzzi, D T Baird, R Webb, and B K Campbell Direct in vivo effects of leptin on ovarian steroidogenesis in sheep Reproduction, December 1, 2004; 128(6): 757 - 765. [Abstract] [Full Text] [PDF]

				N.J. Raine-Fenning, B.K. Campbell, N.R. Kendall, J.S. Clewes, and I.R. Johnson Quantifying the changes in endometrial vascularity throughout the normal menstrual cycle with three-dimensional power Doppler angiography Hum. Reprod., February 1, 2004; 19(2): 330 - 338. [Abstract] [Full Text] [PDF]

				C. Glister, D. S. Tannetta, N. P. Groome, and P. G. Knight Interactions Between Follicle-Stimulating Hormone and Growth Factors in Modulating Secretion of Steroids and Inhibin-Related Peptides by Nonluteinized Bovine Granulosa Cells Biol Reprod, October 1, 2001; 65(4): 1020 - 1028. [Abstract] [Full Text] [PDF]