HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by McMurtry, J P Articles by Brocht, D M Search for Related Content PubMed PubMed Citation Articles by McMurtry, J P Articles by Brocht, D M

The development of a homologous radioimmunoassay (RIA) for chicken insulin-like growth factor-I (cIGF-I) and its use to investigate the developmental changes in IGF-I in the chicken and turkey is described. A doubleantibody RIA has been developed using recombinantly derived cIGF-I as antigen, radiolabelled tracer and standard. The resulting immunoassay has a minimum detection limit of 0·035 ng and effective dose of 2·5 ng. Dose–response curves of chicken and turkey plasma and tissue extracts were parallel with cIGF-I standard. The antiserum is specific for IGF-I as no cross-reactivity with chicken IGF-II, insulin, glucagon, gastrin or avian pancreatic polypeptide was observed. We have also established that acid/ethanol extraction of chicken and turkey plasma reduced possible interference of IGF-binding proteins (IGFBPs) in the RIA. Comparison of IGF-I immunoactivity in unextracted and acid/ethanol-extracted samples following gel filtration under acidic and neutral conditions indicates that the cIGFBPs may be acid-labile. Analyses of samples from growing chickens and turkeys using the homologous avian reagents revealed higher IGF-I concentrations than if the IGF were quantified using heterologous mammalian-derived reagents. A similar pattern was observed when tissue extracts were assayed for IGF-I content. The application of the homologous RIA to monitor blood and tissue IGF-I levels during embryonic development and posthatch growth in avian species will provide more accurate comparisons of results from studies on the role of IGF-I in growth and metabolism of domestic birds.

Journal of Endocrinology (1994) 142, 225–234

This article has been cited by other articles:

				P. K. Sethi, J. P. McMurtry, G. M. Pesti, H. M. Edwards Jr., and S. E. Aggrey Physiological Responses to Divergent Selection for Phytate Phosphorus Bioavailability in a Randombred Chicken Population Poult. Sci., December 1, 2008; 87(12): 2512 - 2516. [Abstract] [Full Text] [PDF]

				M. de Beer, J. P. McMurtry, D. M. Brocht, and C. N. Coon An Examination of the Role of Feeding Regimens in Regulating Metabolism During the Broiler Breeder Grower Period. 2. Plasma Hormones and Metabolites Poult. Sci., February 1, 2008; 87(2): 264 - 275. [Abstract] [Full Text] [PDF]

				S. A. Jenkins, M. Muchow, M. P. Richards, J. P. McMurtry, and T. E. Porter Administration of Adrenocorticotropic Hormone during Chicken Embryonic Development Prematurely Induces Pituitary Growth Hormone Cells Endocrinology, August 1, 2007; 148(8): 3914 - 3921. [Abstract] [Full Text] [PDF]

				J. W. Lu, J. P. McMurtry, and C. N. Coon Developmental Changes of Plasma Insulin, Glucagon, Insulin-like Growth Factors, Thyroid Hormones, and Glucose Concentrations in Chick Embryos and Hatched Chicks Poult. Sci., April 1, 2007; 86(4): 673 - 683. [Abstract] [Full Text] [PDF]

				H. Zhou, C. M. Evock-Clover, J. P. McMurtry, C. M. Ashwell, and S. J. Lamont Genome-Wide Linkage Analysis to Identify Chromosomal Regions Affecting Phenotypic Traits in the Chicken. IV. Metabolic Traits Poult. Sci., February 1, 2007; 86(2): 267 - 276. [Abstract] [Full Text] [PDF]

				O. Halevy, A. Krispin, Y. Leshem, J. P. McMurtry, and S. Yahav Early-age heat exposure affects skeletal muscle satellite cell proliferation and differentiation in chicks Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2001; 281(1): R302 - R309. [Abstract] [Full Text] [PDF]

				C. M. Ashwell, S. M. Czerwinski, D. M. Brocht, and J. P. McMurtry Hormonal regulation of leptin expression in broiler chickens Am J Physiol Regulatory Integrative Comp Physiol, January 1, 1999; 276(1): R226 - R232. [Abstract] [Full Text] [PDF]

				B. A. Watkins, C.-L. Shen, J. P. McMurtry, H. Xu, S. D. Bain, K. G. D. Allen, and M. F. Seifert Dietary Lipids Modulate Bone Prostaglandin E2 Production, Insulin-Like Growth Factor-I Concentration and Formation Rate in Chicks J. Nutr., June 1, 1997; 127(6): 1084 - 1091. [Abstract] [Full Text]