HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lacasa, D Articles by Giudicelli, Y Search for Related Content PubMed Articles by Lacasa, D Articles by Giudicelli, Y

The effects of ovarian status on insulin- and phorbol 12-myristate 13-acetate (TPA)-stimulated lipogenic responses, phorbol ester-specific binding to protein kinase C (PKC) and immunoblot-quantified β- and -PKC isoform levels were compared in female rat fat cells from subcutaneous and deep intra-abdominal (parametrial) fat deposits.

In control rats, the cytosolic PKC content per cell was 70% lower in subcutaneous than in parametrial adipocytes. In subcutaneous and parametrial fat cells, the cytosolic PKC contents were reduced by ovariectomy and restored to normal by the administration of ovarian hormones (oestradiol plus progesterone). However, the lipogenic response to TPA was unaltered by ovarian status in both fat deposits, contrasting with the insulin-stimulated lipogenic response. This response increased in parametrial fat cells after ovariectomy and returned to normal after ovarian hormone treatment whereas, in subcutaneous fat cells, the insulin response was either unaltered by ovariectomy or increased by the administration of ovarian hormones. This study shows important site-related differences in fat cell PKC content but also reveals a similar modulation of this PKC by ovarian status, regardless of the anatomical localization of the fat cells. The physiological significance of these findings is discussed.

Journal of Endocrinology (1994) 140, 275–282

This article has been cited by other articles:

				E. Garcia Dos Santos, M. N. Dieudonne, R. Pecquery, V. Le Moal, Y. Giudicelli, and D. Lacasa Rapid Nongenomic E2 Effects on p42/p44 MAPK, Activator Protein-1, and cAMP Response Element Binding Protein in Rat White Adipocytes Endocrinology, March 1, 2002; 143(3): 930 - 940. [Abstract] [Full Text] [PDF]

				E. Garcia, D. Lacasa, and Y. Giudicelli Estradiol Stimulation of c-fos and c-jun Expressions and Activator Protein-1 Deoxyribonucleic Acid Binding Activity in Rat White Adipocyte Endocrinology, August 1, 2000; 141(8): 2837 - 2846. [Abstract] [Full Text] [PDF]

				M. N. Dieudonne, R. Pecquery, A. Boumediene, M. C. Leneveu, and Y. Giudicelli Androgen receptors in human preadipocytes and adipocytes: regional specificities and regulation by sex steroids Am J Physiol Cell Physiol, June 1, 1998; 274(6): C1645 - C1652. [Abstract] [Full Text] [PDF]

				D. Lacasa, E. Garcia, B. Agli, and Y. Giudicelli Control of Rat Preadipocyte Adipose Conversion by Ovarian Status: Regional Specificity and Possible Involvement of the Mitogen-Activated Protein Kinase-Dependent and c-fos Signaling Pathways Endocrinology, July 1, 1997; 138(7): 2729 - 2734. [Abstract] [Full Text] [PDF]