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An in vitro method of insulin assay based upon the glucose uptake of the isolated rat diaphragm is described and a statistical appraisal of the method given.

The minimum concentration of insulin which was detected by this method was of the order of 10^–4 i.u. of insulin/ml. The index of precision () varied in eighteen studies of regression from 0·18 to 0·74. The slope of the log dose-response curve was found to vary significantly from experiment to experiment.

The results are briefly compared with those obtained with other in vitro methods of insulin assay.