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The mechanism of arachidonic acid (AA)-induced LH release was characterized using sheep pituitary cells in primary culture permeabilized with Staphylococcal -toxin. In intact cells, exogenous AA evoked release of LH in a manner which was partially dependent on extracellular Ca²⁺. At similar concentrations, AA also caused cell permeabilization as monitored by efflux of [³H]2-deoxyglucose metabolites. In -toxin-permeabilized cells where cytosolic Ca²⁺ was clamped at resting levels, AA retained its ability to cause LH release. Unlike the stimulation of exocytosis produced by Ca²⁺, phorbol ester or cyclic AMP, AA-evoked release was independent of ATP and was not inhibited by pretreatment with N-ethyl maleimide. These findings indicated that exogenous AA does not cause LH release by Ca²⁺ influx or mobilization or by activating protein kinase C. The results suggest that LH release induced by exogenous AA is probably due to its detergent-like properties, and does not represent true exocytosis.

Journal of Endocrinology (1992) 132, 77–82

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