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Arginine vasopressin (AVP) stimulates Na⁺K⁺ ATPase and ornithine decarboxylase (ODC) activity in the rat medullary thick ascending limb. The effect of difluoromethyl ornithine (DFMO), a specific inhibitor of ODC activity, on AVP-stimulated Na⁺K⁺ATPase activity was evaluated using a cytochemical bioassay. Peaks in Na⁺K⁺ATPase activity in cultured rat renal segments which occurred after tissue had been exposed to 1 fmol AVP/l were completely inhibited by the addition of 20 mmol DFMO/l to the culture medium containing AVP. The addition of 20 mmol DFMO/l to the culture medium containing AVP in the concentration range 0·001–10 fmol/l inhibited completely the stimulation of Na⁺K⁺ATPase activity by AVP. The response of Na⁺K⁺ATPase to increasing doses of ATP (10–40 g polypeptide/l) was not influenced by the addition of 20 mmol DFMO/l to the culture medium containing AVP, suggesting that the prevention of AVP-stimulated Na⁺K⁺ATPase activity by DFMO was not due to a direct effect on the enzyme.

Journal of Endocrinology (1989) 121, 435–439