| HOME | HELP | CONTACT US | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The androgen dependency of prostatic bud formation in fetal rat urogenital sinuses was studied using brief treatments with androgen, and the incorporation of androgens by the sinus mesenchyme was followed by steroid autoradiography.
Urogenital sinuses from 16·5-day fetuses of both sexes were grown in organ culture and treated with androgens for periods ranging from 4 to 72 h and then transferred to control medium. A minimum treatment of 24 h was required to induce prostatic buds in male sinuses and of 36 h in all female sinuses. This difference in response disappeared after more prolonged treatment. In both sexes the number of prostatic buds increased with the time of exposure to androgens. Prostatic bud formation continued for 24–36 h after transfer to control medium.
Steroid autoradiographic analysis showed that the labelled androgen was concentrated in the mesenchymal nuclei. The rate of incorporation rose steeply during the first 12 h and then more slowly. After transfer to control medium the amount of labelled androgen decreased rapidly to half within 12 h and then decreased more slowly. In the competition experiments a 200-fold excess of unlabelled testosterone or dihydrotestosterone in the labelling medium greatly reduced the nuclear labelling with [3H]testosterone.
J. Endocr. (1986) 110, 467–470
This article has been cited by other articles:
|
|
 |
|
  L. M. Cotton, M. K. O'Bryan, and B. T. Hinton Cellular Signaling by Fibroblast Growth Factors (FGFs) and Their Receptors (FGFRs) in Male Reproduction Endocr. Rev., April 1, 2008; 29(2): 193 - 216. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Pu, L. Huang, L. Birch, and G. S. Prins Androgen Regulation of Prostate Morphoregulatory Gene Expression: Fgf10-Dependent and -Independent Pathways Endocrinology, April 1, 2007; 148(4): 1697 - 1706. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. D. Abbott, T.-M. Lin, N. T. Rasmussen, R. M. Albrecht, J. E. Schmid, and R. E. Peterson Lack of Expression of EGF and TGF-{alpha} in the Fetal Mouse Alters Formation of Prostatic Epithelial Buds and Influences the Response to TCDD Toxicol. Sci., December 1, 2003; 76(2): 427 - 436. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Woodham, L. Birch, and G. S. Prins Neonatal Estrogen Down-Regulates Prostatic Androgen Receptor through a Proteosome-Mediated Protein Degradation Pathway Endocrinology, November 1, 2003; 144(11): 4841 - 4850. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Thomson, B. G. Timms, L. Barton, G. R. Cunha, and O. C. Grace The role of smooth muscle in regulating prostatic induction Development, March 6, 2003; 129(8): 1905 - 1912. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | CONTACT US | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
